Question 1: Which forensic technique is most suitable for identifying a person using dental records?
- A. Odontology
- B. Palynology
- C. Entomology
- D. Toxicology
Answer
Answer: A. Odontology
Explanation: Forensic odontology involves the analysis of dental evidence, such as teeth patterns, for human identification.
Question 2: The Becke line test is used in forensic science to determine:
- A. Refractive index
- B. DNA profile
- C. Blood type
- D. Hair structure
Answer
Answer: A. Refractive index
Explanation: The Becke line test helps determine the refractive index of transparent materials like glass or fibers under a microscope.
Question 3: Which drug is known as the “date rape drug”?
- A. Cocaine
- B. GHB
- C. Heroin
- D. Methamphetamine
Answer
Answer: B. GHB
Explanation: Gamma-Hydroxybutyrate (GHB) is a central nervous system depressant commonly referred to as a “date rape drug.”
Question 4: Which chemical is commonly used for the development of latent fingerprints on non-porous surfaces?
- A. Ninhydrin
- B. Superglue (Cyanoacrylate)
- C. Silver nitrate
- D. Amido Black
Answer
Answer: B. Superglue (Cyanoacrylate)
Explanation: Cyanoacrylate fuming reacts with moisture in latent prints, creating a white polymer visible on non-porous surfaces.
Question 5: In forensic anthropology, which bone is most commonly used to estimate stature?
- A. Femur
- B. Radius
- C. Humerus
- D. Ulna
Answer
Answer: A. Femur
Explanation: The femur is the longest bone in the human body, making it the most reliable for estimating height.
Question 6: The luminol test is used to detect:
- A. Gunshot residue
- B. Blood
- C. Seminal fluid
- D. Hair
Answer
Answer: B. Blood
Explanation: Luminol reacts with hemoglobin to produce a blue chemiluminescence, indicating the presence of blood.
Question 7: Which type of evidence is considered the most reliable for human identification?
- A. Dental records
- B. DNA
- C. Fingerprints
- D. Blood type
Answer
Answer: B. DNA
Explanation: DNA provides unique genetic profiles for individual identification, making it the most reliable form of forensic evidence.
Question 8: The term “chain of custody” refers to:
- A. Procedures for arresting a suspect
- B. Documentation of evidence handling
- C. Protocols for crime scene photography
- D. Steps in autopsy examination
Answer
Answer: B. Documentation of evidence handling
Explanation: Chain of custody ensures proper documentation of evidence transfer and storage to maintain its integrity.
Question 9: What is the primary purpose of a forensic autopsy?
- A. Determine cause of death
- B. Identify the deceased
- C. Collect toxicological evidence
- D. All of the above
Answer
Answer: D. All of the above
Explanation: A forensic autopsy aims to determine cause and manner of death, identify the deceased, and collect evidence.
Question 10: The Controlled Substances Act categorizes drugs based on:
- A. Medical use and abuse potential
- B. Cost and accessibility
- C. Manufacturer
- D. Route of administration
Answer
Answer: A. Medical use and abuse potential
Explanation: Drugs are classified into schedules under the Controlled Substances Act based on their medical use and potential for abuse.
Question 11: Which method is used to measure the alcohol content in blood?
- A. Gas chromatography
- B. Spectrophotometry
- C. PCR
- D. Electrophoresis
Answer
Answer: A. Gas chromatography
Explanation: Gas chromatography is the most accurate method to measure blood alcohol concentration in forensic analysis.
Question 12: In forensic toxicology, the term “LD50” refers to:
- A. Lowest dose of a substance
- B. Dose lethal to 50% of a population
- C. Maximum safe dose
- D. Median effective dose
Answer
Answer: B. Dose lethal to 50% of a population
Explanation: LD50 indicates the dose of a substance that is lethal to 50% of the test population, used in toxicology studies.
Question 13: What is the main function of the mitochondrial DNA in forensic analysis?
- A. Individual identification
- B. Maternal lineage tracing
- C. Determining age
- D. Predicting eye color
Answer
Answer: B. Maternal lineage tracing
Explanation: Mitochondrial DNA is inherited maternally and is useful in tracing maternal lineage, especially when nuclear DNA is degraded.
Question 14: Which technique is best for the analysis of gunshot residue (GSR)?
- A. UV Spectroscopy
- B. Thin Layer Chromatography
- C. Scanning Electron Microscopy (SEM)
- D. Gas Chromatography
Answer
Answer: C. Scanning Electron Microscopy (SEM)
Explanation: SEM coupled with Energy Dispersive X-ray Spectroscopy (EDX) provides detailed morphology and elemental composition of GSR particles.
Question 15: Which protein is primarily targeted in the confirmatory test for blood?
- A. Hemoglobin
- B. Myoglobin
- C. Albumin
- D. Fibrinogen
Answer
Answer: A. Hemoglobin
Explanation: Confirmatory tests for blood target hemoglobin, the oxygen-carrying protein in red blood cells.
Question 16: Which of the following is a presumptive test for saliva?
- A. Phadebas test
- B. Takayama test
- C. Teichmann test
- D. Duquenois-Levine test
Answer
Answer: A. Phadebas test
Explanation: The Phadebas test detects the presence of amylase, an enzyme found in saliva, making it a common presumptive test.
Question 17: Which method is used to determine post-mortem interval using insects?
- A. Forensic pathology
- B. Forensic anthropology
- C. Forensic entomology
- D. Forensic odontology
Answer
Answer: C. Forensic entomology
Explanation: Forensic entomology uses the study of insect life cycles and succession to estimate the time since death.
Question 18: Which light source is most effective in visualizing latent prints on multicolored surfaces?
- A. UV light
- B. Blue light
- C. Infrared light
- D. Laser light
Answer
Answer: A. UV light
Explanation: Uv light can enhance the fluorescence of treated latent prints, especially on complex backgrounds.
Question 19: The technique used for species identification through DNA is called:
- A. DNA fingerprinting
- B. DNA barcoding
- C. STR analysis
- D. Y-STR typing
Answer
Answer: B. DNA barcoding
Explanation: DNA barcoding uses a short genetic marker from a standardized region to identify species.
Question 20: Which test is used for detecting nitrate residues in gunshot cases?
- A. Kastle-Meyer test
- B. Greiss test
- C. Benzidine test
- D. Duquenois-Levine test
Answer
Answer: B. Greiss test
Explanation: The Greiss test is a colorimetric test used to detect nitrate compounds in gunshot residue.
Question 21: What is the primary forensic use of diatoms?
- A. Determining time of death
- B. Estimating age
- C. Diagnosing poisoning
- D. Drowning investigation
Answer
Answer: D. Drowning investigation
Explanation: Presence of diatoms in the organs of a drowned body helps confirm drowning as cause of death.
Question 22: What is the full form of NIBIN?
- A. National Integrated Ballistics Information Network
- B. National Investigation of Biometric Identification Network
- C. National Inventory of Bullet Imaging Network
- D. National Institute of Ballistic and Imaging Network
Answer
Answer: A. National Integrated Ballistics Information Network
Explanation: NIBIN is used in the U.S. for comparing ballistic evidence to link crimes involving firearms.
Question 23: Which pigment is responsible for the greenish discoloration in decomposing bodies?
- A. Melanin
- B. Hemoglobin
- C. Biliverdin
- D. Sulphaemoglobin
Answer
Answer: D. Sulphaemoglobin
Explanation: The greenish discoloration observed during the early stages of putrefaction is mainly due to the formation of sulphaemoglobin. This pigment results from the interaction of hydrogen sulphide (produced by intestinal bacteria) with haemoglobin. It typically appears in the lower right abdomen, where putrefaction begins. While biliverdin can also cause greenish tints, sulphaemoglobin is the primary cause in decomposing bodies.
Question 24: What is the primary basis of forensic palynology?
- A. Bone density
- B. Pollen and spores
- C. Tooth eruption
- D. Skull morphology
Answer
Answer: B. Pollen and spores
Explanation: Forensic palynology is the study of pollen and spores to connect a suspect or object to a particular location or time.
Question 25: Which test is used for detecting marijuana?
- A. Duquenois-Levine test
- B. Marquis test
- C. Scott test
- D. Liebermann test
Answer
Answer: A. Duquenois-Levine test
Explanation: The Duquenois-Levine test is a color test used to detect the presence of marijuana.
Question 26: What part of a hair is most useful for nuclear DNA analysis?
- A. Cuticle
- B. Cortex
- C. Medulla
- D. Root
Answer
Answer: D. Root
Explanation: The root of the hair contains nucleated cells which are essential for nuclear DNA analysis.
Question 27: What is the ideal method to visualize tool marks on metallic surfaces?
- A. UV light
- B. Magnetic powder
- C. Oblique lighting
- D. Cyanoacrylate fuming
Answer
Answer: C. Oblique lighting
Explanation: Oblique lighting reveals surface details and striations on metal surfaces, making it useful for tool mark analysis.
Question 28: Which type of fingerprint pattern has no delta?
- A. Loop
- B. Whorl
- C. Arch
- D. Tented Arch
Answer
Answer: C. Arch
Explanation: Arch patterns typically lack deltas and cores, distinguishing them from loops and whorls.
Question 29: Which element is commonly found in primer residue?
- A. Calcium
- B. Zinc
- C. Lead
- D. Iron
Answer
Answer: C. Lead
Explanation: Primer residue often contains lead, barium, and antimony from the ignition compound.
Question 30: What is the main use of capillary electrophoresis in forensic DNA analysis?
- A. DNA extraction
- B. DNA quantification
- C. DNA amplification
- D. DNA separation
Answer
Answer: D. DNA separation
Explanation: Capillary electrophoresis is used for separating amplified DNA fragments based on size during STR analysis.
Question 31: Which method is commonly used to preserve digital evidence?
- A. Hashing
- B. Cloning
- C. Encryption
- D. Formatting
Answer
Answer: B. Cloning
Explanation: Cloning creates an exact bit-by-bit copy of a digital device, preserving the original evidence.
Question 32: What is the purpose of luminol in forensic investigations?
- A. Detect latent fingerprints
- B. Identify drug residues
- C. Reveal blood traces
- D. Analyze DNA
Answer
Answer: C. Reveal blood traces
Explanation: Luminol reacts with the iron in hemoglobin to produce a luminescent glow, revealing latent blood traces.
Question 33: In forensic accounting, what does ‘red flag’ indicate?
- A. A legal audit
- B. Suspicious activity
- C. Tax compliance
- D. Budget approval
Answer
Answer: B. Suspicious activity
Explanation: A red flag refers to a warning sign of potential fraud or financial irregularity.
Question 34: What does the acronym AFIS stand for?
- A. Automated Fingerprint Identification System
- B. Advanced Forensic Imaging Software
- C. Automated Facial Identification System
- D. Authentication Fingerprint Information Server
Answer
Answer: A. Automated Fingerprint Identification System
Explanation: AFIS is a biometric identification system that stores and compares fingerprint data for identification purposes.
Question 35: Which bone is most commonly used in stature estimation?
- A. Femur
- B. Tibia
- C. Humerus
- D. Radius
Answer
Answer: A. Femur
Explanation: The femur is the longest and strongest bone and provides reliable data for estimating an individual’s height.
Question 36: What is the role of EDTA in blood collection tubes?
- A. Stabilizer
- B. Coagulant
- C. Preservative
- D. Anticoagulant
Answer
Answer: D. Anticoagulant
Explanation: EDTA binds calcium ions, preventing blood clotting and preserving the sample for analysis.
Question 37: Which organ is typically first to show post-mortem changes?
- A. Brain
- B. Liver
- C. Heart
- D. Kidneys
Answer
Answer: B. Liver
Explanation: Due to its high enzyme content and central metabolic role, the liver often shows early autolytic changes after death.
Question 38: What is the main application of the Teichmann test?
- A. Confirming semen presence
- B. Detecting drugs
- C. Identifying blood crystals
- D. Analyzing ink
Answer
Answer: C. Identifying blood crystals
Explanation: The Teichmann test forms hemin crystals in the presence of blood, used as a confirmatory test for blood.
Question 39: Which forensic technique uses radioisotopes for analysis?
- A. Gas Chromatography
- B. Mass Spectrometry
- C. Neutron Activation Analysis
- D. UV Spectroscopy
Answer
Answer: C. Neutron Activation Analysis
Explanation: This technique irradiates samples with neutrons and measures resulting gamma rays to identify elements.
Question 40: Which of the following disciplines focuses on bite mark analysis?
- A. Forensic Entomology
- B. Forensic Odontology
- C. Forensic Serology
- D. Forensic Ballistics
Answer
Answer: B. Forensic Odontology
Explanation: Forensic odontologists analyze dental evidence including bite marks for identification purposes.
Question 41: Which technique is best suited for detecting invisible ink on documents?
- A. UV light examination
- B. Infrared photography
- C. Thin Layer Chromatography
- D. Electrostatic detection
Answer
Answer: A. UV light examination
Explanation: Many invisible inks fluoresce under UV light, revealing hidden writing.
Question 42: What is the main component analyzed in breath alcohol testing?
- A. Ethanol
- B. Methanol
- C. Acetone
- D. Propanol
Answer
Answer: A. Ethanol
Explanation: Breath analyzers detect ethanol levels to estimate blood alcohol concentration (BAC).
Question 43: What does ridge ending refer to in fingerprint analysis?
- A. Start of a ridge
- B. End of a ridge line
- C. A dot on the fingerprint
- D. Fork in a ridge line
Answer
Answer: B. End of a ridge line
Explanation: Ridge endings are minutiae points where the friction ridge ends, used for identification.
Question 44: Which test is used for detecting semen in forensic examination?
- A. Takayama test
- B. Acid phosphatase test
- C. Kastle-Meyer test
- D. Benzidine test
Answer
Answer: B. Acid phosphatase test
Explanation: This test detects high levels of acid phosphatase, an enzyme found in seminal fluid.
Question 45: Which of the following is a confirmatory test for blood?
- A. Luminol test
- B. Kastle-Meyer test
- C. Takayama test
- D. Acid phosphatase test
Answer
Answer: C. Takayama test
Explanation: The Takayama test produces hemochromogen crystals, confirming the presence of blood.
Question 46: What kind of evidence is hair without follicular tag?
- A. Class evidence
- B. Individual evidence
- C. Trace evidence
- D. Exculpatory evidence
Answer
Answer: A. Class evidence
Explanation: Hair without the root or follicle cannot be linked to an individual with certainty and is considered class evidence.
Question 47: What is the function of a comparison microscope?
- A. To enlarge bullets
- B. To analyze paint
- C. To compare side-by-side samples
- D. To identify ink types
Answer
Answer: C. To compare side-by-side samples
Explanation: Comparison microscopes allow simultaneous viewing of two samples, useful in bullet and fiber comparisons.
Question 48: What type of fracture is most common in cases of child abuse?
- A. Spiral fracture
- B. Greenstick fracture
- C. Comminuted fracture
- D. Transverse fracture
Answer
Answer: B. Greenstick fracture
Explanation: Greenstick fractures are incomplete fractures commonly seen in children due to the flexibility of their bones and are often indicators of abuse when found in unusual locations.
Question 49: Which technique is commonly used for age estimation in teeth?
- A. DNA methylation
- B. Cementum annulation
- C. Root transparency
- D. Radiocarbon dating
Answer
Answer: B. Cementum annulation
Explanation: Cementum annulation involves counting the growth layers in tooth cementum, which correlate with age, making it a reliable age estimation technique.
Question 50: What is the role of mitochondrial DNA in forensic analysis?
- A. It determines gender
- B. It identifies species
- C. It is used in maternal lineage tracing
- D. It reveals genetic disorders
Answer
Answer: C. It is used in maternal lineage tracing
Explanation: Mitochondrial DNA is inherited maternally and is especially useful in degraded samples where nuclear DNA is not available, allowing identification through maternal relatives.

